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Video 2. Translocation of a chimaera of protein kinase C and green fluorescent protein
Video 2: Translocation of a chimaera of protein kinase C and green fluorescent protein. Fluorescence from a protein kinase C-green fluorescent protein chimaera, recorded simultaneously with Video 1. The chimaera, which is initially distributed uniformly through the cytoplasm (but not the nuclei), translocates to the plasma membrane when the cells are stimulated with agonist. Every time cytosolic calcium increases, the chimaera moves to the membrane, moving off again each time calcium concentrations fall.
